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CyFlow Space

  • Configurations with up to 13 fluorescence parameters and 3 physical light scattering parameters
  • Choice of 488, 638, 640, 405, 532, 561, 365, 375, 594 nm lasers
  • Maximum acquisition rate up to 25,000 signals/s
  • Scatter particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: < 100 MESF (FITC), < 50 MESF (PE)


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CyFlow Space – its flexibility gives you the space you need for your work

The CyFlow Space is a high performance multi-laser flow cytometer system which offers the most flexible, simple and reliable features for routine and research work.

It´s a compact multi-colour instrument for analysis of individual cells and microscopic particles in suspension. The advanced flow cytometry technology of the CyFlow Space covers a wide range of different applications.

Fluorescence excitation by 1 to 5 light sources covering a range from ultra-violet to red excitation lines. Analysis of up to 13 fluorescence parameters and 3 physical light scattering parameters from near UV to infra-red light. Analysis of cell and particle concentration by True Absolute Volumetric Counting (TVAC). Cell sorting by an optional cell and particle sorter. Fixed standard configurations and flexible customized configurations tailored for the user´s requirement. Walk-away automation by sample auto-loading from 96- and 384-well plates.

Optional: CyFlow Space Autoloading Station

Optional: CyFlow Space Sorter Module 



Light Sources:

  • Up to 5 light sources
  • Blue solid state laser: 50mW fix or 200mW adjustable 488nm
  • Red diode laser: 25mW 638nm or 40mW 640nm
  • Violet diode laser: 100mW 405nm
  • Green DPSS laser: 30 or 100mW 532nm
  • Yellow laser: 100mW 561nm
  • High power UV LED: 365nm
  • Ultra-violet diode laser: 60mW 375nm
  • Orange diode laser: 50mW 594nm


  • Modular optical system with up to 16/8 optical parameters with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL13
  • Exchangeable optical filters
  • Colour CCD camera for sample flow monitoring
  • Light polarization module (upon request)

 Flow System:

  •  Quartz glass flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer controlled precision syringe pump speed continuously adjustable from 0-20 µl/sec.


  •  Microsoft Windows™ operating system with full network support
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
  • Storage of instrument settings, screen layout templates, panels, gates and compensation matrices
  • Automated reporting of statistics, graphics and instrument settings to Word or Excel


  • L 560mm x W 650mm x H 300mm





CyFlow Space Autoloading Station

General Facts:

  • (Automatic sample loading from 96/384 well plates)
  • High speed mode < 15 minutes/96 well plates
  • User defined cleaning and sample re-suspension
  • Start-up time < 2 minutes


  • 96 and 384 well-plates with V, U and flat-boom wells
  • Plates meeting the Standards (ANSI/SLAS 1-2004 through 4-2004)

 Analysed Volume:

  • Flexible choice of sample volume 10 to 150µl


  • Sample injection speed adjustable from 0.2µl/sec. to 10µl/sec.
  • Cycle time per microplate
  •    a. High speed mode < 15min
  •    b. Balanced mode < 30min
  •    c. Precision mode < 50 min
  •    or customised by operator


  • CyPAD Windows™ based software


  • L 200mm x W 320mm x H 280mm





Partec FloMax flow cytometry Data Acquisition and Analysis Software

Windows™ based Partec FloMax software includes multiple online gating, logical gates, and colour gating. N-colour online and offline software compensation is another standard feature of FloMax software. Data may be acquired with compensation or as uncompensated raw data. Either way, compensation can be done, undone, or changed after analysis on stored data. User-selectable statistics are calculated on subset populations identified by regions that are created by the user with easy-to-use drawing tools and combined using simple, logical formulas. Other sophisticated features include cell cycle analysis and peak analysis. Custom reports are quickly and easily generated from the acquired data using the integrated, macro-driven interface with Microsoft™ Word and Excel. FloMax data files are stored in FCS standard, and therefore, they are fully compatible with other commercial flow cytometry software.

FloMax software is pre-installed on the Windows™ PC that is delivered as part of the CyFlow Space systems. Three additional licenses for offline copies of FloMax® software are also included free of charge, thus offering a cost-effective solution for working groups with the need for offline data analysis software.

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