CyFlow Cube 6

  • Configurations with up 6 optical parameters (up to 4 colours)
  • 488 nm blue laser and 638 nm red laser
  • Particle size: 0.1 – 100 µm
  • Fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Optional CyFlow Robby 6 Autoloading Station

Compact and economic flow cytometer

The CyFlow Cube 6 is a compact benchtop flow cytometer for the analysis of individual cells and microscopic particles. Different CyFlow Cube 6 configurations to select from offer optimal solutions for dedicated applications. The easy-to-use CyFlow software provides control of the instrument,  data acquisition, analysis and storage. CyFlow Cube 6 offers True Volumetric Absolute Counting (TVAC), which allows the display of particle concentrations for any subsets of cells without the need for reference beads, even if defined by a gate at a later time after the acquisition.

Highlights

Highlights

  • High-performance, bench-top design with built-in PC and a 15.4" TFT monitor
  • 20mW@488nm and 25mW@638nm lasers
  • Superior fluorescence sensitivity: ≤ 100 MESF (FITC) | ≤ 50 MESF (PE)
  • Superior nanotechnology: small particle detection < 50 nm
  • Optional CyFlow Robby autoloader for well plates and tubes
  • Unique price/performance ratio
Software

Software

CyFlow: Intuitive software for CyFlow Cube 6

The newly designed, intuitive CyFlow acquisition and analysis software offers the measurement of millions of cells at your fingertips.

  • Quickly choose your plots for analysis from all of the combinations shown in Preview Mode.
  • Easily compare plots with the Overlay Wizard, for single or dual parameters.
  • Share and customise your results within your research organisation with the Report Generator.
  • Change your view with Zoom and V-Log Scale; you are even able to gate in Zoom Mode.
  • Compensate rapidly with the Compensation Wizard.
  • CyFlow optimally supports the True Volumetric Absolute Counting of the CyFlow Cube 6, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.
Specifications

Specifications

Light Sources:

  • Up to 2 simultaneous light sources (laser)
  • o   Blue laser: 50mW @488nm
  • o   Red laser: 25mW @638nm

 

Optics:

  • Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL4
  • Exchangeable optical filters

 

Flow System:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Computer controlled precision syringe pump, speed continuously adjustable from 0-20 µl/sec.

 

Software:

  • Windows™ based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy experimental template set up (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data

 

Dimensions:

  • Standalone instrument:
    L 385mm x W 280mm x H 290mm
  • With Autoloading Station: L 745mm
Upgrades

Upgrades

CyFlow Robby 6 Autoloading Station

General facts:

  • Protective housing with no light incidence 
  • Flexible well/tube measurements

 

Microplates and tubes:

  • 48- or 96-well plates; V-, U- and flat-bottom are fitting (max. 2)
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

 

Sample volume:

  • Flexible choice of sample volume up to 180 µL for plates and 2,000 µL for tubes

 

Speed:

  • Continuously adjustable from 0.2 µL/s to 10 µL/s
  • Approx. 1h for a 96-well plate (speed 2 µL/s, sample volume 20 µL)

 

Software:

  • CyFlow Windows™-based software

 

Dimensions:

  • W 360mm x H 270mm x D 370mm
Applications

Applications

Absolute cell and particle counting
Cell and particle sorting

Microbiology:

  • Yeast viability and fermentation process control 
  • Water quality analysis 
  • Absolute counting of bacteria and other microorganisms 
  • Viability of bacteria and other microorganisms
  • Quality control in dairy industry and milk products 
  • Marine biology and algae 
  • Agrosciences and aquaculture 
  • Quality control in cosmetics

 

Industrial applications: 

  • Bioreactor process optimisation 
  • Quality assurance in food and beverage industry 
  • Biomonitoring 
  • Biofuels

 

Cell biology: 

  • Cell cycle and DNA analysis 
  • Viability and live/dead analysis 
  • Cell proliferation 
  • Apoptosis 
  • Cancer research 
  • Toxicology 
  • Cytokines and bead-based assays 
  • Cell signalling 
  • Plant biology
  • Detection of ploidy level 
  • Plant genome size and DNA analysis 
  • Biomedical research 
  • Animal models 
  • Stem cells 
  • Immunology 
  • Haematological disorders 
  • Platelets
Kontakt

Sysmex Suisse AG

+41 44 718 38 38
info@sysmex.ch
Tödistrasse 50
8810 Horgen
Schweiz

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