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CyFlow Ploidy Analyser

Ploidy determinations with the CyFlow Ploidy Analyser are performed by the precise measurement of the total DNA content of individual nuclei extracted and stained with Partec CyStain reagents, which can be derived from almost any plant or animal tissue. When used in combination with Partec CyStain PI reagents, the relative genome size of plant and animal nuclei can also be determine on the CyFlow Ploidy Analyser.

The CyFlow Ploidy Analyser measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4” colour display. The automated, real-time ploidy analysis is extremely time- and cost- efficient.

Features

CyView: flow cytometry data acquisition and analysis software

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Technical Specifications
Light sources Up to two light sources
UV LED (365 nm)
Nd-YAG green laser at 532 nm (30 mW)
Optics Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)
Flow system Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
Completely closed fluidic system
Sample port with biosafety cleaning system
Software Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
Easy set-up of experimental templates (configuration files)
Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Peak analysis function
Dimensions Stand-alone instrument: W 385mm x H 290mm x D 280mm
With Autoloading Station: W 745mm
With screen open: H 528mm
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