This internationally awarded, truly stand-alone Flow Cytometer is ultra-compact and uniquely designed for all applications in cell analysis and absolute counting. With the CyFlow Cube 8, accurate flow cytometry is offered by employing 8 optical parameters within an instrument size of only 50x47x35.5 cm. Forward Scatter (FSC) and Side Scatter (SSC) work in combination with 6 fluorescence channels (FL1-FL6). An optional, built-in CyFlow Sorter module for diamond-piezo-based, closed, non-destructive and non-hazardous cell and particle sorting is available.

Features

CyFlow: Intuitive software for CyFlow Cube 8

The newly designed, intuitive CyFlow acquisition and analysis software offers the measurement of millions of cells at your fingertips.

Quickly choose your plots for analysis from all of the combinations shown in Preview Mode.
Easily compare plots with the Overlay Wizard, for single or dual parameters.
Share and customise your results within your research organisation with the Report Generator.
Change your view with Zoom and V-Log Scale; you are even able to gate in Zoom Mode.
Compensate rapidly with the Compensation Wizard.
CyFlow optimally supports the True Volumetric Absolute Counting of the CyFlow Cube 8, displaying particle concentrations for any subsets of cells, even if defined by a gate at a later time after the acquisition.

Technical Specifications

Light sources	Up to four light sources (three lasers + high-power UV LED) Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm Red laser: 25mW at 638nm, 40mW at 640nm Violet laser: 100mW at 405nm UV laser: 60mW at 375nm Green laser: 30mW at 532nm, 100mW at 532nm Yellow laser: 100mW at 561nm Orange laser: 50mW at 594nm High-power UV LED: 365nm
Optics	Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL6 Exchangeable optical filters
Flow system	Quartz flow cuvette for laminar sample transport and hydrodynamic focussing True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s
Software	Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display Easy set-up of experimental templates (configuration files) Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Dimensions	Stand-alone instrument: W 500mm x H 370mm x D 470mm With Autoloading Station: W 840mm
CyFlow Cube 8 Sorter
General facts	Configurations with up to five optical parameters (up to three colours). Sterile sorting by closed fluidic system. Optional CyFlow Robby 8 Autoloading Station for well plates and tubes
Light sources	Up to two simultaneous light sources (lasers)> Blue laser: 50mW at 488nm, adjustable to 200mW at 488nm Red laser: 25mW at 638nm, 40mW at 640nm Violet laser: 100mW at 405nm UV laser: 60mW at 375nm Green laser: 30mW at 532nm, 100mW at 532nm Yellow laser: 100mW at 561nm Orange laser: 50mW at 594nm
Optics	Modular optical system with selected PMTs with integrated electronic preamplifier for FSC, SSC, FL1-FL3 Exchangeable optical filters
Flow system	Quartz flow cuvette for laminar sample transport and sorting process Completely closed and integrated fluidic system True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement Computer-controlled precision syringe pump, speed continuously adjustable from 0-20 µL/s
Software	Windows™-based FCM software CyFlow for real-time data acquisition, real-time data analysis and real-time data display Easy set-up of experimental templates (configuration files) Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Dimensions	W 500mm x H 370mm x D 470mm (without sorting module)